MILLIPORE 3025 SAMPLING MANIFOLD LIQUID BIOBURDEN CFU PLATE COUNTING FILTER TEST

You are bidding on a MILLIPORE 3025 SAMPLING MANIFOLD LIQUID BIOBURDEN CFU PLATE COUNTING FILTER TESTING as can be seen in the pictures! 

Cosmetically this unit is in very good condition with one small chip on the right side as can be seen in the pictures we took, please check carefully. This small crack does not affect operation whatsoever but we thought important to mention and show.

Applications

Bioburden testing of filterable products is performed to determine the number of viable and reproducible microorganisms. By recovering organisms on and in a membrane filter, processing efficiency and the microbial quality of a sample can be evaluated to assure that the product is not harmful to the intended recipient.

Detecting spoilage microorganisms are critical for foods and beverages as they may be responsible for the deterioration of sensory qualities (visual, texture, and taste) and may potentially render the product harmful for consumption. Using membrane filtration, detection of these spoilage microorganisms keep contaminated items from reaching the market.

Monitoring for compliance to drinking water standards using Membrane Filtration Technique, determines if there are levels of pathogenic microorganisms in treated water or wastewater. These microorganisms include coliforms such as Escherichia coli (E. coli) and Legionella which are both classified as pathogens.

"A unique microbiology manifold that allows you to optimize testing without sacrificing cleanliness. 

With interchangeable components and coupling devices, the manifold takes direction from you.

The manifold is designed to fit most laboratory autoclaves by separating into manageable components.

This MILLIPORE 3021 manifold can be easily separated for disinfection and/or sterilization.

Whether you are right or left handed, setting up your test workflow is second nature. The components interchangeable and designed with a simple slip fit to the manifold.

For filtration, mount receptacle of filter holding assembly on a …suitable device such that a pressure differential (34 kPa to 51 kPa) can be exerted on the filter membrane.”

- Standard Methods for the Examination of Water and Wastewater, 22nd Edition, 9222B (f).

The following Related Products below which ARE NOT included with this Millipore Membrane Sampling Manifold but may be necessary:

- MicroFunnel Filter Funnels

- MicroFunnel Plus and Plus AP Filter Funnels

- MicroCheck® Beverage Monitors

- 47 mm Magnetic Filter Funnels

- Sentino Filter Funnels

- Sentino Filter Dispenser and Membranes

- S-pack Membranes

- Forceps

- Petri Dishes

- Ampoule Media

“For filtration, mount receptacle of filter holding assembly on a ...suitable device such that a pressure differential (34 kPa to 51 kPa) can be exerted on the filter membrane.” - Standard Methods for the Examination of Water and Wastewater, 22nd Edition, 9222B (f).

To clean this Laboratory Manifold a 70% IPA, 5% Hypochlorite solution (bleach) or 30% Hydrogen peroxide should be used but contact time for these solutions should not exceed 10 minutes.

A volume of reagent that is equal to or greater than the volume of the product being filtered is the recommended volume of cleaning agent to run through each port. 

You can use a sporicidal more frequently than once a month, this will not affect the manifold. Select a sporicidecthat best suites your needs, either a 5-7% hypochlorite solution or a 30% hydrogen peroxide followed by a water rinse is recommended.

Introduction

Application and Intended Use

The membrane filter (MF) technique is a method of analyzing samples for microbial contamination. An aqueous solution is pulled through a membrane filter and the filter is placed on microbiology growth media then incubated. After a prescribed incubation period, colony forming units (CFUs) are counted and reviewed for appropriate morphology.

Bioburden testing of filterable products is performed to determine the number of viable and reproducible microorganisms. By recovering the microorganisms on and in a membrane filter, sterilization effectiveness can be calculated and raw materials are released to assure that the product is not harmful to the intended recipient.

Detecting spoilage microorganisms is critical for food and beverages as they are responsible for the deterioration of the sensory quality, visual changes and potentially render the product harmful to the intended recipient. By using membrane filtration, the recovery and detection of the spoilage microorganisms will keep the contaminated items from reaching the market.

Monitoring for the compliance of drinking water standards via plate counting (filtering), determines if there are levels of pathogenic microorganisms in treated water or wastewater. These microorganisms include coliforms such as Escherichia coli (E. coli) and Legionella which are both classified as pathogens.

This Laboratory Manifold is uniquely designed for an easy adaptability to your microbiological method for the filtration of aqueous solutions by MF technique. The manifold features convenient interchangeable parts to attach to any standard laboratory vacuum system. Thus providing maximum processing while maintaining compliance to regulatory standards.

Use of personal protective equipment (PPE) is strongly recommended when preparing, operating and maintaining Laboratory Manifolds. PPE may include items such as eye protection, hearing protection, sterile gloves, heat tolerant gloves, or chemically resistant gloves.

Preparing System for Use & Maintenance

This Laboratory Manifold is provided non-sterile. It is recommended to follow the steps below to aseptically prepare the manifold for testing of microbiological samples:

1. Rinse the manifold and all the components of the manifold with deionized (DI) or purified water to remove any large debris on the exterior and interior.

2. Use autoclave paper or autoclave bags, ensure the components and manifold will be protected from non-sterile air when removing from the autoclave.

3. Place inside of an autoclave in a validated load pattern to maximize the sterilization of the manifold and components.

4. Autoclave the manifold and components at a temperature between 121 – 123 °C (250 - 253 °F) at 1.0 bar (100 kPa, 15 psi) for 10 - 15 minutes.

5. After autoclaving is complete, allow all pieces to cool to a safe handling temperature or use personal protective equipment (PPE) to remove. Carefully remove the manifold and components ensuring to maintain the integrity of the autoclave paper/wrapping.

6. Store sterile manifold and components in a secure location to prevent breach of protective wrapping until manifold and components are ready for testing.

It is recommended to follow the steps below on a monthly basis, at minimum, to disinfect the manifold for testing microbiological samples.

1. Rinse the manifold and all the components of the manifold with deionized (DI) or purified water in a sufficient volume to remove any large debris on the exterior and interior.

2. With the vacuum turned off, fill the manifold with approximately 155 mL of disinfectant of choice. The amount used should be enough to fill the manifold, valve stems and valve assembly. Please see disinfectant instructions to determine the amount of contact time.

3. After disinfectant hold, rinse manifold from the adaptor with DI or purified water. Manifold valves are to be left in the open position to allow for air drying.

Additional Recommended Cleaning Methods:

1. Spray manifold components with water solution of 5% bleach, wait 10 minutes and rinse with water.

2. Spray manifold components with water solution of 35% hydrogen peroxide, wait 10 minutes and rinse with water.

3. Spray manifold components with water solution of 70% IPA, wait 24 hours and rinse with water

The instructions above for disinfection and sterilization of this Laboratory Manifold are the manufacturing recommendations however,  please follow your internal procedures and risk assessments for disinfection and sterilization.

*NOTE: The use of Micro90 cleaning solution is not recommended as this will remove the anodization of the aluminum components.

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Stock # 211701