100% TA INSTRUMENTS NANO ITC 2g MODEL 5300 CALORIMETER FREESHIP

This listing is for a 100% 90-day-Warranty: TA INSTRUMENTS NANO ITC 2g MODEL 5300 CALORIMETER as can be seen in the pictures. 

IMPORTANT: This TA INSTRUMENTS NANO ITC 5300 needs the accessory computer and hardware which should include the Instrumental Control Software, this unit does not need nor include the software and will work perfectly so long as the accessory hardware user has or should have does include the Software installed in it!

Model: TA Instruments Nano ITC 2g Model 5300 Calorimeter 

Manufacturer: TA Instruments

Type: Calorimeter

Condition: This fully tested TA Instruments Nano ITC 2g Model 5300 Calorimeter IS GUARANTEED to perform at factory specifications and that's why we offer a 90-DAY-WARRANTY on this unit!!!

These TA Instruments Nano ITC 2g Model 5300 Calorimeter cost a pretty penny and this one is in Great Condition!

Picture is actual item buyer will receive.

TECHNICAL INFORMATION IN ITALICS BLUE BELOW WAS TAKEN FROM THE WEB:

tainstruments.com/nano-itc/

DESCRIPTION

The Nano ITC Standard Volume and Nano ITC Low Volume isothermal titration calorimeters are designed to provide maximum sensitivity and flexibility for the study of biomolecular binding. Both instruments use solid state thermoelectric heating and cooling systems to precisely control temperature, and have the same flexible injection syringe assemblies for efficient and accurate delivery of titrant. The true isothermal power compensation design of the Nano ITC instruments provides the highest sensitivity and flexibility for an ultrasensitive ITC analyzing biological samples in-solution.

Cell Geometry Fixed Cylindrical Fixed Cylindrical

Cell Composition 24K / Hastelloy 24K

Active Cell Volume 1.0 mL 190 µL

Operating Temperature Range 2 °C to 80 °C 2 °C to 80 °C

Minimum Detectable Heat 0.1 µJ 0.05 µJ

Maximum Measureable Heat 5,000 µJ 5,000 µJ

Low Noise Level 0.0025 µW 0.0014 µW

Baseline Stability 0.02 µW/hr 0.02 µW/hr

Response Time 13 sec / 18 sec 11 sec

Injection Syringe Volume 100 µL and 250 µL 50 µL

Minimum Injection Volume 0.12 µL / 0.26 µL 0.06 µL

Stirring Speed Range 0 to 400 rpm 0 to 400 rpm

The NANO ITC features many of the high performance

technologies found in the Affinity ITC. It is a versatile, highsensitivity, cost-effective isothermal titration calorimeter that

can easily outperform competitive systems in a wide range

of applications.

Features and Benefits:

• Choice of Standard Volume (1.0 mL) or Low Volume (190 µL) cells

• Solid-state active heating and cooling for true isothermal temperature control

• High precision injection buret for accurate titrant delivery

• Unique removable injection syringe for fast, reliable loading and cleaning

• Powerful ITCRun and NanoAnalyze for the most comprehensive suite of tools for

method optimization, model fitting, batch analysis, graphing and data export

More Technical information on the TA INSTRUMENTS NANO can be found online at:

tainstruments.com/pdf/brochure/BROCH-MICRO-EN.pdf

The Nano ITC cell is optimized in shape, material, and volume

to provide the greatest measurement accuracy over the

widest range of sample chemistries.

Choice of Cell Volumes:

The Nano ITC features two fixed-in-place calorimetric cells: a sample cell where injections

take place and a matching reference cell. Two cell volumes are available: 1.0 mL

(Standard Volume) and 190 µL (Low Volume).

Selection of cell volume depends on the range of binding constants to be measured

(Kd: mM to low nM) and the availability of sample. TA Instruments’ experienced application

teams can recommend the best instrument configuration for your specific measurement

requirements.

Cylindrical Cell Geometry:

The cylindrical cell geometry maximizes stirring efficiency, eliminates dead zones and

entrapped air bubbles, which are common in competitive designs.

Cell Composition:

To maximize measurement accuracy and response, the Nano ITC Standard Volume

configuration has cells constructed of either 24 k 99.999% Gold (Au0) or Hastelloy. The

Low volume configuration is only available with the Gold (Au0) cells. The inert chemical

properties of Gold, its high thermal conductivity and its ability to be cleaned with strong

acids and bases make it the preferred choice for ultrasensitive ITC instruments.

Applications:

Low and Standard Volume Comparison

The sensitivity of the Nano ITC Low Volume ensures that with less sample the instrument will generate

accurate and reproducible results in a shorter overall titration time. The Standard Volume and Low

Volume Nano ITC instruments provide the flexibility and sensitivity for performing a wide variety of ITC

experiments.

Characterizing Binding Interactions by ITC

All binding events are accompanied by the evolution or absorption of heat (a change

in enthalpy, ΔH). In a single ITC experiment a full thermodynamic characterization of

the binding reactions can be obtained. With the appropriate experimental design,

fundamental information about the molecular interactions driving the process, as well

as the stoichiometry of binding (n) and the binding constant (Kd) is generated. The first

figure shows a typical incremental titration (20, 5 μL injections) of an inhibitor, 2’-CMP,

titrated into RNase A; n 0.99, Kd 5.92x10-7 M, and ΔH -64.4 kJ mol-1. The second figure

shows the same experiment, plotting the individual integrated peak areas vs the ratio

of the two binding molecules. As the binding sites become saturated, the amount of

heat produced with individual injections decreases. The resulting titration curve reveals

valuable information on the enthalpy (ΔH), entropy (ΔS) and overall Gibbs free energy

(ΔG0) of the reaction taking place in the calorimeter. ITC is a powerful analytical tool and

considered the most sensitive assay technique for characterizing the fundamental driving

forces of molecular binding reactions.

Protein Interactions

When two proteins interact and bind, conformational changes in the proteins, and

rearrangement of the solvent in the vicinity of the binding site, result in the absorption

or generation of heat. Quantification of this reaction heat by ITC provides a complete

thermodynamic description of the binding interaction, the stoichiometry of binding, and

the association constant. This figure contains the titration data of porcine pancreatic

trypsin into soybean trypsin inhibitor using a Nano ITC. Twenty, 5 µL aliquots of ligand were

titrated into the sample cell while the temperature of the system was maintained at 25 °C.

Top panel: The signal (heat) produced following each addition of protein to the inhibitor.

Bottom panel: Integration of the heats over the time course of the experiment; the µJ in

each peak are plotted against the mole ratio of the titrant to inhibitor.

Characterization of Enzyme Kinetics

Every reaction generates or absorbs heat, so every reaction can in principle be studied

by calorimetry. In practice it has been shown that representative enzymes from every EC

classification can be analyzed kinetically using ITC. In addition, ITC analyses are rapid,

precise, nondestructive, compatible with both physiological and synthetic substrates, and

are as sensitive as spectroscopic techniques but do not require a spectroscopic label

or chemical tag. Importantly, ITC analyses of enzyme kinetics are also straightforward.

The figure shows the hydrolysis of a single 10 µL injection of trypsin into a solution of BAEE

in the absence (blue) and presence (red) of benzamidine, a competitive inhibitor. The

area under both curves (representing the total heat output for complete conversion of

substrate to product) is the same either in the presence or absence of inhibitor, allowing

the KM and kcat of the reaction under both conditions to be calculated, as well as the

inhibition constant.

Continuous Single Injection

Continuous single injection titration is an attractive alternative to the traditional incremental

titration ITC for samples exhibiting very rapid binding reactions. These continuous injection

experiments can be completed in less total time than normally required for a full set of

incremental titrations. This technique provides accurate determinations of stoichiometry

(n) and enthalpy (ΔH) for a wide range of binding constants. Continuous injection and

incremental injection experiments can be performed in both the ITC standard volume

and low volume instruments with no alterations in hardware or software supplied with the

instruments.

The Nano ITC utilizes multiple solid-state thermoelectric elements for active

heating and cooling of the sample and reference cells. A unique removable

buret and injection syringe ensures easy sample loading and accurate

sample delivery.

Accurate Temperature Control with Active Heating and Cooling:

• Faster heating and cooling between temperature set points

• Rapid equilibration at temperature set point

• Active temperature control eliminates drift on long ITC experiments

The absorption or evolution of heat as a result of a binding reaction is detected by the thermoelectric

elements after which heater power is adjusted to maintain the temperature difference between the sample

and reference cell at zero. The combination of power compensation and thermoelectric temperature control

ensures the fastest response and highest resolution for ITC.

Unique Injection Buret and Removable Injection Syringe:

• Accurate control of the titrant volume delivery and user-selectable stir speed is accomplished with a

unique, easily removed buret

• Removable injection syringes allows thorough cleaning and easy sample loading.

• Partial syringe fills for short titrations are user-programmable in the instrument control software.

All of our equipment is fully tested to perform at factory specifications before being shipped.

Used and in excellent working order.

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Stock # 232307